A brief history of the application of Bacillus subtilis in agriculture

 1.The discovery of Bacillus subtilis

Bacillus is the first probiotic group that people have come into contact with and researched, and Bacillus subtilis is one of the bacteria of high concern among Bacillus.



In 1835, Ehrenberg discovered Bacillus subtilis and named it "Vibrio subtilis".

In 1872, with the establishment of the bacterial classification system, Cohn named it "Bacillus subtilis".

In 1945, Johnson et al reported that Bacillus subtilis has the effect of preventing and controlling plant diseases;

In 1997, Kunst F. et al first completed the complete genome sequencing of Bacillus subtilis and published the results in the journal Nature


So far, in the field of planting, Bacillus subtilis has been widely used to control plant diseases, promote growth, improve soil structure, and improve crop quality.


2. Screening and production of subtilis

Traditional strain screening is usually based on morphological characteristics, enzyme-producing ability, bacteriostatic ability and stress resistance analysis and other methods to isolate in animal intestine, plant rhizosphere, soil environment, fermentation body and other environments, and carry out molecular biology, physiological and biochemical analysis. identification to screen out strains with excellent performance. The production of Bacillus subtilis includes fermentation and granulation. There are three main fermentation processes used in industrialization: liquid fermentation, solid-state fermentation and liquid-solid two-phase fermentation. In actual production, liquid fermentation is the main method. Granulation is an important post-processing link for fermentation of microbial products, which is of great significance for ensuring the stability of the bacterial agent and subsequent storage and transportation. High temperature, vacuum, freezing, radiation, etc. are usually used in the granulation process. Studies have reported that a large number of bacterial cells die during this process. The current technological conditions are spray drying and freeze drying.


3. The application of subtilis in the direction of microbial pesticides

As a biocontrol bacteria, Bacillus subtilis has a very broad antibacterial spectrum and has inhibitory effects on a variety of fungal and bacterial diseases: such as sheath blight, root rot, stem rot, total rot, fusarium wilt, verticillium wilt Disease, bacterial wilt, etc., can also control downy mildew, gray mold, powdery mildew, blight, apple mold heart disease, citrus penicillium, nectarine brown rot, strawberry gray mold, crown rot, anthracnose, Black spot, canker, golden pear fruit rot. Poplar canker, rot, black spot and anthracnose, tea leaf spot, tobacco anthracnose, black shank, red star fungus, root rot, cotton blight, fusarium wilt and other more than 100 kinds of diseases.


If you have any interest on Bacillus subtilis, please feel free to contact us:

Anne chou

Email:anne@cyclebioscience.com

Whatsapp:+86 15050351426

评论

发表评论

此博客中的热门博文

How to prevent discoloration of tomato sepals

图片
Recently, many vegetable farmers have reported that the quality of tomato sepals are poor and turned brown, so that the fruit does not look bright, which affects the sales price, and many vegetable farmers have no remedy for this. There are four main reasons for the poor quality of tomato sepals. Vegetable farmers can take preventive measures. 1. Root injury is growing poorly Due to drought, waterlogging, excessive fertilization, etc., the roots are damaged, the plant growth is poor, and the plant's endogenous hormone synthesis is insufficient, which will cause the leaves to yellow and the sepals of the fruit will age and turn yellow-brown. This requires strengthening management, improving growth, and avoiding root-damaging factors. Suggest Irrigation with rooting agents to promote root recovery, foliar spray with 6000 times of sodium nitrophenolate and 1500 times of brassinolide plus 400 times of nucleotides to promote the green synthesis of tomato leaves and sepals. 2. High tempe
阅读全文

Foliar spraying of Alginate oligosaccharides restores growth of wheat under chlorpyrifos stress

图片
    Chlorpyrifos, as an organophosphorus insecticide and acaricide, is suitable for the control of various chewing and sucking mouthparts pests such as wheat. However, excessive use will lead to changes in crop soluble proteins, glutathione-S-transferase, glutathione peroxidase, etc., resulting in pesticide stress, thereby affecting crop growth. In 2015, Zhang Shoudong, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, etc. published an article "Effects of Alginate Oligosaccharides on Physiological and Biochemical Indexes of Wheat Seedlings Under Chlorpyrifos Stress". In this experiment, spraying 0.4% concentration of Alginate oligosaccharides significantly alleviated the decrease of chlorophyll content and wheat biomass in wheat seedlings under chlorpyrifos stress, inhibited the increase of proline, soluble protein and soluble sugar content, and enhanced wheat Resistance to chlorpyrifos stress. Test Results: (Note: CK: distilled water treatment, T0-T4: 0/0
阅读全文

Analysis on the role of rhizobia in soybean planting

图片
Rhizobium soybean is a live microbial preparation. The rhizobia in the root nodules and the legumes are mutually beneficial: the organic matter produced by the legumes through photosynthesis is partly supplied to the rhizobia; the ammonia produced by the rhizobia through biological nitrogen fixation is supplied to the legumes plant. , it fixes free nitrogen in the air to supply soybeans to meet soybean demand for nitrogen. It is a good measure to increase soybean yield, save fertilizer and reduce pollution, and has been valued by researchers around the world. First, the role of rhizobia 1. Nitrogen fixation Soybean rhizobia uses the products of soybean photosynthesis as energy, and converts nitrogen in the air into nutrients required for soybean growth and development. Rhizobium and soybean have a symbiotic nitrogen fixation relationship. At the beginning, soybean rhizobia fixed little nitrogen, until flowering to drum. The initial stage of grain is the peak of nitrogen fixation by so
阅读全文