Application of proline in enhancing stress resistance of plants

 Proline is an important amino acid accumulated in plants under stress such as drought, high temperature, high salt, freezing, ultraviolet irradiation and heavy metal, and plays a key role in plant osmotic regulation.

 

(1)Proline can reduce the damage of salt stress to plants

In 1 x Hoagland nutrient solution culture muskmelon seedling, the growth of normal (figure 1, A), add 100mmol·L-1NaCl in 1 x Hoagland nutrient solution melon seedling plants grow bad, in short, the salt injury symptoms of more severe (figure 1, B), and in the add 100mmol·L-1NaCl + 0.2mmol·L - 1proline of 1 x Hoagland nutrient solution melon seedling growth basic normal, the salt injury symptoms significantly reduce (figure 1, C).

(2) Proline can effectively improve the morphological indexes of rice under salt stress

From table 3-2, figure 3-3 (A and F), the salt stress significantly inhibit rice seedling stage of seedling height, root length, root number and the relative seedling height and relative root number, seedling height, root length, fell by 3.83 cm and 0.75 cm respectively than control, the root number reduce 4 and 6, relative seedling height and relative root number is 40.5% and 31.7% according to the normal, but had no significant inhibitory effect on the relative root length.

 

Under the condition of salt stress, proline seeding could significantly improve the seedling height, root length, root number and their relative values of germinated rice seeds. Moreover, under the treatment of low concentration of proline 5mmol/L (FIG. 3-3G), the effect of salt stress on phenotypic traits of rice could be alleviated, and even the root length, relative seedling height and relative root length could be restored to the normal level.

Higher concentration of proline (30mmol/L) could restore the seedling height to the normal level (FIG. 3-3i).

The results showed that proline could effectively improve the morphological indexes of rice under salt stress.

(3) Proline participates in plant flowering signal transduction

Proline plays an important role in plant growth and development as a signal molecule.

Many studies have shown that proline plays a certain role in flower development of plants.

The content of free proline was the highest in arabidopsis thaliana, especially in pollen grains, accounting for 26% of the total amino acid content.

Through the proline physiological toxicity experiments of arabidopsis thaliana, observed 45mmol·L for proline L can inhibit the growth of wild-type arabidopsis, led to the deaths of young plants, in different concentrations (0, 15, 30, 45 mmol·L) proline medium, Sow for 2 weeks, kao2 all show the resistance of proline, embodied in the root and the growth of aboveground part were significantly better than that of wild type (figure 1), plant fresh weight and chlorophyll content were higher than in wild type (figure 2).

If you have any interest, please feel free to contact:

Anne Chou

Email:anne@cyclebioscience.com

Whatsapp:+86 15050351426